Electroporation Inefficiency in Caulobacter henricii CB4

Abstract

Caulobacter is a well-studied model organism. The introduction of plasmids into Caulobacter by way of electroporation or conjugation has been well-documented, as well as the barrier Caulobacter’s S-layer has played in the efficiency of these techniques. Standard electroporation and conjugation protocols with Caulobacter henricii CB4 resulted in zero transformants. Various parameters have been shown to increases electroporation efficiency and these parameters were adjusted for CB4. Although, CB4 was not able to take up foreign DNA though electroporation or conjugation, a variety of parameters which increases electroporation efficiency are now located in one location.