Rectification of artificial molecular recombination with the use of high fidelity enzyme in the amplification of 16S rDNA sequences from Stool sample.

Vijay Nema

Abstract


Reliance on routinely used taq polymerases for amplification may generate spurious sequences, especially in metagenomic studies utilizing complex mixtures of various DNA templates. Use of high fidelity enzymes and verification of the sequences using various software tools before submission to the databases ensures better quality and confidence.


Keywords


Metagenomics; 16S rDNA; Sequencing; Chimera; High fidelity taq polymerase; Pintail.

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