Inability of UV spectrophotometric and RP-HPLC methods to perform as stability indicating assay method for the quantification of eugenol

Kannissery Pramod, Shahid Hussain Ansari, Javed Ali

Abstract


Availability of a stability indicating assay method for carrying out accelerated stability studies of drug products is essential. In a search of a suitable stability indicating assay method for the quantification of eugenol UV spectrophotometric and high pressure liquid chromatography (HPLC) methods were tried. Forced degradation studies included use of eugenol samples from acid induced, base induced, oxidative degradation, thermal and photolytic degradations. UV spectrophotometry using methanol as solvent and RP-HPLC method using using photo diode array detector was studied. The UV spectrophotometric and high pressure liquid chromatography (HPLC) methods failed to demonstrate itself as a stability indicating method as shown by the results of forced degradation studies. From the study results we concluded that UV spectrophotometric and HPLC methods could not be used for the stability studies of eugenol.

Keywords


forced degradation studies, acid induced degradation, base induced degradation, oxidative degradation, thermal degradation, photolytic degradation

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