Valorization of orange (Citrus sinensis) peel waste: Isolation and structural elucidation of polymethoxylated flavones

David Díaz Díaz, Jesús G. Díaz, Laura Chacón Cruz

Abstract


This study explores the valorization of orange (Citrus sinensis) peel waste as a source of bioactive
polymethoxylated flavones (PMFs) with nutraceutical potential. A dichloromethane extract from fresh orange
peel (including both albedo and flavedo) was subjected to sequential chromatographic separation, yielding three
known compounds: 3,5,6,7,8,3′,4′-heptamethoxyflavone (1), nobiletin (2), and sinensetin (3). Structural
elucidation was achieved through 1D and 2D NMR techniques, supported by high-resolution mass spectrometry.
Although these compounds are already known, their recovery from citrus waste underscores the potential of agri-
food by-products as valuable sources of health-promoting natural products. The findings contribute to sustainable
waste valorization and support the development of high-value ingredients for pharmaceutical and nutraceutical
applications

Keywords


Citrus sinensis, orange peel, polymethoxylated flavones, nobiletin, sinensetin, NMR spectroscopy.

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